Marcin Wawrzyniak

Isolation of cells from blood and tissues: PBMC`s, sinus tissue cells, tonsil mononuclear cells, epithelial cells from lungs

Cell culture techniques of immune and tissue cells: T cells, epithelial cells from lung and sinus, keratinocytes in monolayer and air-liquid interface cultures and co-culture of immune and tissue cells

Assessment of epithelial cells integrity for cells grown in air-liquid interface cultures

Immunofluorescence staining of cells and tissues – confocal microscopy and data analysis with Bitplane Imaris software

RNA and DNA isolation from cells and tissue, expression analysis with qRT-PCR methods

Immunoenximatic tests (ELISA) and multiplex immunoassay
Next generatation RNA sequencing analysis with the use of MetaCore software

Designing of staining panels for multicolor analysis for intracellular, intranuclear and surface staining for flow cytometry, data acquisition and analysis – Flowjo, Kaluza software

Cells sorting with cytokine secretion assays and culture of rare cells

Magnetic cells isolation and separation, cell sorting with FACS devices (ARIA III)

Animal Experiments with mouse models - Animal handing and sacrifice, tissue isolation and processing, isolation of cells from tissues.

Data analysis and representation with Microsoft Office, Graphpad Prism, Adobe Illustrator, CorelDraw

Experience in writing scientific publications as a medical writer

Member of European Academy of Allergy and Clinical Immunology

FELASA Certificate – Education and Training of Persons Conducting Animal Experiments

Computer-operating knowledge of PC computers with Windows environment, Office
Software, CorelDraw, Adobe Illustrator, Kaluza, FlowJo, Bitplane Imaris, GraphPad Prism,
EndNote