пропозиції роботи
Katarzyna Wawrzyniak-Turek
Biotechnologist, Project Manager
Warszawa,
mazowieckie
Dołącz do GoldenLine i obejrzyj pełny profil tej użytkowniczki
Zobacz pełny profil
Umiejętności
Biotechnologia
Praca w zespole
Zarządzanie projektami
Badania i Rozwój
spektrofotometria UV/VIS
Spektroskopia fluorescencyjna
Solid-phase synthesis (DNA and RNA)
SELEX (in vitro selection)
Elektroforeza żelwa (PAGE, SDS-PAGE, IEF)
In vitro transcription
Języki
polski
ojczysty
angielski
biegły
francuski
podstawowy
niemiecki
podstawowy
Doświadczenie zawodowe
R&D Project Manager
Coordinating collaborative R&D project, co-financed by NCBiR and NFOŚiGW:
- coordination of the work of research teams in a framework of a 4-members consortium;
- supervision over the R&D process (consulting research methodology, implementing new milestones based on the obtained results, approving the research reports, coordinating contacts between subcontractors);
- responsible for project’s promotion at scientific events;
- project budget maintenance and its documentation management.
Fundraising for SMEs:
- Entrepreneurs support in the implementation of competitive and innovative concepts – responsible for grant applications for R&D and implementation projects;
- Developing projects' concepts and budgets;
- Application with success within Smart Growth, Poland East and Regional Operational Programs (POIR, POPW, RPO).
- coordination of the work of research teams in a framework of a 4-members consortium;
- supervision over the R&D process (consulting research methodology, implementing new milestones based on the obtained results, approving the research reports, coordinating contacts between subcontractors);
- responsible for project’s promotion at scientific events;
- project budget maintenance and its documentation management.
Fundraising for SMEs:
- Entrepreneurs support in the implementation of competitive and innovative concepts – responsible for grant applications for R&D and implementation projects;
- Developing projects' concepts and budgets;
- Application with success within Smart Growth, Poland East and Regional Operational Programs (POIR, POPW, RPO).
Science Writer
Bio-Tech Media Sp. z o.o.
writting articles for the polish biotechnology web-portal (biotechnologia.pl)
Visiting Researcher
Lund University (Biochemistry and Structural Biology, Department of Chemistry), Sweden
Testing of modified siRNA activity in human MCF-7 cells.
- siRNA transfection
- luciferase assay
- siRNA transfection
- luciferase assay
Research Scientist
Max Planck Institute for Biophysical Chemistry, Göttingen, Niemcy
Research projects conducted in the field of functional nucleic acids (natural and artificial aptamers, riboswitches and deoxyribozymes):
1) Development of a new combinatorial mutagenesis method to study functional RNA and DNA
2) In vitro selection (SELEX) of fluorogenic aptamers
3) Biochemical characterization of deoxyribozymes
4) Molybdenum Cofactor riboswitch (Moco RNA) - structural probing and ligand binding assays
- Projects planning and realization - results of several completed projects published in recognizable
journals (e.g. Nature, Chemical Communications)
- Supervision of university students projects
- Scientific presentations at international conferences
- Collaborating with other reasearch teams (e.g. from Lund University)
Techniques used:
- automated solid-phase synthesis of RNA and DNA
- RNA and DNA sequencing
- fluorescent and UV-vis spectroscopy
- SELEX (in vitro selection from DNA and RNA libraries)
- HPLC (analytical, preparative, RP)
- gel electrophoresis (PAGE, SDS-PAGE, agarose)
- molecular cloning
- PCR, RT-PCR, in vitro transcription and translation
- SHAPE and DMS probing, enzymatic probing assays
- 32P labeling, handling of radioactive material
1) Development of a new combinatorial mutagenesis method to study functional RNA and DNA
2) In vitro selection (SELEX) of fluorogenic aptamers
3) Biochemical characterization of deoxyribozymes
4) Molybdenum Cofactor riboswitch (Moco RNA) - structural probing and ligand binding assays
- Projects planning and realization - results of several completed projects published in recognizable
journals (e.g. Nature, Chemical Communications)
- Supervision of university students projects
- Scientific presentations at international conferences
- Collaborating with other reasearch teams (e.g. from Lund University)
Techniques used:
- automated solid-phase synthesis of RNA and DNA
- RNA and DNA sequencing
- fluorescent and UV-vis spectroscopy
- SELEX (in vitro selection from DNA and RNA libraries)
- HPLC (analytical, preparative, RP)
- gel electrophoresis (PAGE, SDS-PAGE, agarose)
- molecular cloning
- PCR, RT-PCR, in vitro transcription and translation
- SHAPE and DMS probing, enzymatic probing assays
- 32P labeling, handling of radioactive material
Leonardo da Vinci fellow (Intern)
INRA (French National Institute for Agricultural Research), Clermont - Ferrand (Francja)
Fellowship within the Leonardo da Vinci European Programme
Trainee in Proteomics Centre
Investigation of the aorta proteome in a specific nutritional model in mice (reaserach project: Nutritional Prevention of Atherosclerosis by Omega 3 Polyunsaturated Fatty Acids)
• Preparation of protein extracts from mouse aorta
• Two-dimensional electrophoresis for proteomic analysis
• Use of the SameSpots Progenesis software for gel image analysis and for statistics
• Sampling of protein spots on 2-DE gels for mass spectrometry analysis
Trainee in Proteomics Centre
Investigation of the aorta proteome in a specific nutritional model in mice (reaserach project: Nutritional Prevention of Atherosclerosis by Omega 3 Polyunsaturated Fatty Acids)
• Preparation of protein extracts from mouse aorta
• Two-dimensional electrophoresis for proteomic analysis
• Use of the SameSpots Progenesis software for gel image analysis and for statistics
• Sampling of protein spots on 2-DE gels for mass spectrometry analysis
Student trainee
Institute for Neurosciences of Montpellier, Francja
• Maintaining cells in culture
• Western-blot
• Immunofluorescence
• Western-blot
• Immunofluorescence
Erasmus scholarship (Intern)
Ecole Nationale Superieure de Chimie de Montpellier (National Graduate School of Chemistry), Francja
Research project in organic chemistry
• Synthesis, identification and purification of functionalized phosphorus compounds with biological activities (potentially anticancer and antiviral)
• Clasical and microwave organic synthesis
• Analyses using technical apparatus: 1H, 13C and 31P NMR, Automatic Flash Chromatography, GC/MS system, Microwave reactor
• Results were presented at 18th International Conference on Phosphorus Chemistry (poster session)
• Synthesis, identification and purification of functionalized phosphorus compounds with biological activities (potentially anticancer and antiviral)
• Clasical and microwave organic synthesis
• Analyses using technical apparatus: 1H, 13C and 31P NMR, Automatic Flash Chromatography, GC/MS system, Microwave reactor
• Results were presented at 18th International Conference on Phosphorus Chemistry (poster session)
Szkolenia i kursy
"Industrial and intelectual property rights - patent course"
Organizator: BASF, Gottingen (2015)
"Project management",
Organizator: Golin Wissenschaftsmanagement, Hamburg (2012)
"English Presentation Skills",
Organizator: Gottingen University (2012)
"Scientific Writing",
Organizator: Golin Wissenschaftsmanagement, Hamburg (2014)
"Career planning for PhD students",Organizator: Golin Wissenschaftsmanagement, Hamburg (2012)
Organizator: BASF, Gottingen (2015)
"Project management",
Organizator: Golin Wissenschaftsmanagement, Hamburg (2012)
"English Presentation Skills",
Organizator: Gottingen University (2012)
"Scientific Writing",
Organizator: Golin Wissenschaftsmanagement, Hamburg (2014)
"Career planning for PhD students",Organizator: Golin Wissenschaftsmanagement, Hamburg (2012)
Edukacja
Chemistry (Nucleic Acid Chemistry), doktorat
Georg-August-Universität Göttingen
Biotechnology (Pharmaceutical Biotechnology and Bioinformatics), magisterskie
Politechnika Wrocławska
Specjalizacje
Badania i rozwój
Farmaceutyka/Biotechnologia
Badania i rozwój
Zarządzanie badaniami i rozwojem
Zainteresowania
Aktywny wypoczynek (wspinaczka skałkowa, rower, bieganie, pływanie, narty)
Podróże
Podróże
Inne
PUBLICATIONS:
"Crystal structure of a DNA catalyst"
Published: Nature 2016
Authors: Almudena Ponce-Salvatierra, Katarzyna Wawrzyniak-Turek, Ulrich Steuerwald, Claudia Höbartner, Vladimir Pena
----------------------------------------------
"Deoxyribozyme-mediated ligation for incorporating EPR spin labels and reporter groups into RNA"
Published: Methods in Enzymology 2014
Autors: Katarzyna Wawrzyniak-Turek and Claudia Höbartner
----------------------------------------------
"Enzymatic combinatorial nucleoside deletion scanning mutagenesis of functional RNA"
Published: Chemical Communications 2014
Katarzyna Wawrzyniak-Turek and Claudia Höbartner
----------------------------------------------
"Synthesis of spin-labeled riboswitch RNAs using convertible nucleosides and DNA-catalyzed RNA ligation"
Published: Bioorganic & Medicinal Chemistry 2013
Authors: Lea Buttner, Jan Seikowski, Katarzyna Wawrzyniak, Anne Ochmann, Claudia Höbartner
----------------------------------------------
ADDITIONAL ACTIVITY:
2012
Organizacja sympozjum Nucleic Acid Metal Interactions (Georg-August-Universität Göttingen)
2006-2009
Wychowawca kolonijny na obozach dla dzieci i młodzieży, Biuro Turystyki Aktywnej KOMPAS, Gdańsk
2007, 2008
Pomoc przy organizacji Dolnośląskiego Festiwalu Nauki (pokazy chemiczne)
2008-2009
Wolontariuszka podczas ogólnopolskich i międzynarodowych zawodów w rajdach extremalnych
- Bergson Winter Challenge (Extreme Adventure Race)
- Mistrzostwa Polski w Adventure Racing
Praca sezonowa: Firma 4F (sprzedawca), Call Centre, korepetytorka (chemia)
CONFERENCE PRESENTATIONS
24-28/08/2014
XXI Round Table on Nucleosides, Nucleotides and Nucleic Acids (Chemical Biology of Nucleic Acids), Poznań, Polska
"Combinatorial nucleotide deletion analyzed by primer extension: probing sequenc requirements in functional RNA and DNA" (K. Wawrzyniak-Turek and C. Höbartner)
19-20/09/2013
Nukleinsäurechemie-Treffen, Greifswald, Niemcy
"Combinatorial deletion scanning mutagenesis of functional nucleic acids" (K. Wawrzyniak-Turek, B. Samanta, C. Höbartner)
11-16/06/2013
The 18th annual meeting of the RNA Society, Davos, Szwajcaria
"Spin-labeled cytidine nucleotides in long synthetic RNAs: solid-phase synthesis, post-synthetic labeling, ligation and EPR-spectroscopic characterization"
23-25/05/2013
Joint workshop of the Emerging Fields Initiative “Medicinal Redox Inorganic Chemistry” and the IRTG1422 “Metal Sites in Biomolecules: Structures, Regulation and Mechanisms”, Homberg, Niemcy
"On folding and function of natural and artificial functional nucleic acids" (K. Wawrzyniak-Turek, C. Höbartner)
24-28/04/2012
International Symposium on RNA-based Regulation & Ribosome Biogenesis, Frankfurt am Main, Niemcy
"Probing functional nucleotides in RNA-ligating deoxyribozymes" (K. Wawrzyniak, F. Wachowius, F. JavadiZarnaghi, C. Höbartner)
11-13/04/2012
Joint workshop of the “Dynamics of Electron Transfer Processes within Transition Metal Sites in Biological and Bioinorganic Systems” and the “Metal Sites in Biomolecules: Structures, Regulation and Mechanisms” groups, Hoffgeismar, Niemcy
"RNA folding and ligand binding of a bacterial riboswitch candidate involved in molybdenum cofactor metabolism" (K. Wawrzyniak, C. Höbartner)
6/03/2012
Women's Careers and Networks Symposium, Göttingen, Niemcy
3/11/2011
Women's Careers and Networks Symposium, Göttingen, Niemcy
11-15/06/2010
The 18th International Conference on Phosphorus Chemistry (ICPC 2010), Wrocław
"Working towards the synthesis of new phosphorus nucleoside analogues" (B. Dayde, K. Wawrzyniak, O. F. Muñoz, Jean-Luc Pirat and David Virieux)
"Crystal structure of a DNA catalyst"
Published: Nature 2016
Authors: Almudena Ponce-Salvatierra, Katarzyna Wawrzyniak-Turek, Ulrich Steuerwald, Claudia Höbartner, Vladimir Pena
----------------------------------------------
"Deoxyribozyme-mediated ligation for incorporating EPR spin labels and reporter groups into RNA"
Published: Methods in Enzymology 2014
Autors: Katarzyna Wawrzyniak-Turek and Claudia Höbartner
----------------------------------------------
"Enzymatic combinatorial nucleoside deletion scanning mutagenesis of functional RNA"
Published: Chemical Communications 2014
Katarzyna Wawrzyniak-Turek and Claudia Höbartner
----------------------------------------------
"Synthesis of spin-labeled riboswitch RNAs using convertible nucleosides and DNA-catalyzed RNA ligation"
Published: Bioorganic & Medicinal Chemistry 2013
Authors: Lea Buttner, Jan Seikowski, Katarzyna Wawrzyniak, Anne Ochmann, Claudia Höbartner
----------------------------------------------
ADDITIONAL ACTIVITY:
2012
Organizacja sympozjum Nucleic Acid Metal Interactions (Georg-August-Universität Göttingen)
2006-2009
Wychowawca kolonijny na obozach dla dzieci i młodzieży, Biuro Turystyki Aktywnej KOMPAS, Gdańsk
2007, 2008
Pomoc przy organizacji Dolnośląskiego Festiwalu Nauki (pokazy chemiczne)
2008-2009
Wolontariuszka podczas ogólnopolskich i międzynarodowych zawodów w rajdach extremalnych
- Bergson Winter Challenge (Extreme Adventure Race)
- Mistrzostwa Polski w Adventure Racing
Praca sezonowa: Firma 4F (sprzedawca), Call Centre, korepetytorka (chemia)
CONFERENCE PRESENTATIONS
24-28/08/2014
XXI Round Table on Nucleosides, Nucleotides and Nucleic Acids (Chemical Biology of Nucleic Acids), Poznań, Polska
"Combinatorial nucleotide deletion analyzed by primer extension: probing sequenc requirements in functional RNA and DNA" (K. Wawrzyniak-Turek and C. Höbartner)
19-20/09/2013
Nukleinsäurechemie-Treffen, Greifswald, Niemcy
"Combinatorial deletion scanning mutagenesis of functional nucleic acids" (K. Wawrzyniak-Turek, B. Samanta, C. Höbartner)
11-16/06/2013
The 18th annual meeting of the RNA Society, Davos, Szwajcaria
"Spin-labeled cytidine nucleotides in long synthetic RNAs: solid-phase synthesis, post-synthetic labeling, ligation and EPR-spectroscopic characterization"
23-25/05/2013
Joint workshop of the Emerging Fields Initiative “Medicinal Redox Inorganic Chemistry” and the IRTG1422 “Metal Sites in Biomolecules: Structures, Regulation and Mechanisms”, Homberg, Niemcy
"On folding and function of natural and artificial functional nucleic acids" (K. Wawrzyniak-Turek, C. Höbartner)
24-28/04/2012
International Symposium on RNA-based Regulation & Ribosome Biogenesis, Frankfurt am Main, Niemcy
"Probing functional nucleotides in RNA-ligating deoxyribozymes" (K. Wawrzyniak, F. Wachowius, F. JavadiZarnaghi, C. Höbartner)
11-13/04/2012
Joint workshop of the “Dynamics of Electron Transfer Processes within Transition Metal Sites in Biological and Bioinorganic Systems” and the “Metal Sites in Biomolecules: Structures, Regulation and Mechanisms” groups, Hoffgeismar, Niemcy
"RNA folding and ligand binding of a bacterial riboswitch candidate involved in molybdenum cofactor metabolism" (K. Wawrzyniak, C. Höbartner)
6/03/2012
Women's Careers and Networks Symposium, Göttingen, Niemcy
3/11/2011
Women's Careers and Networks Symposium, Göttingen, Niemcy
11-15/06/2010
The 18th International Conference on Phosphorus Chemistry (ICPC 2010), Wrocław
"Working towards the synthesis of new phosphorus nucleoside analogues" (B. Dayde, K. Wawrzyniak, O. F. Muñoz, Jean-Luc Pirat and David Virieux)
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