Joanna Cygan

Managing of physicochemical properties analytics team
Developing, qualification, transfers of analytical methods
Working on CQA, QTPP, IPC control strategy
Empower SME

Development, qualification, validation and transfer of analytical methods used for proteins
Contact with external suppliers

Physico-chemical analysis of monoclonal antibodies.
Physico-chemical method development for QC.
Peptide mapping using LC-MS/MS methods for proteins and monoclonal antibodies.
Glycan analysis using LC-MS/MS methods and HILIC-HPLC.
Fluorescent labelling of proteins and glycans.
Ellman's test for monoclonal antibodies.
Using Edman degradation to sequencing amino acids in proteins.
Biological tests as CDC.
Gel filtration based on Sepharose, Sephadex beds.
Affinity/RP/NP/HIC/SEC/WCX Chromatography mainly for proteins, peptides, monoclonal antibodies.
HPLC methods - analytical and preparative on Agilent systems.
Technical service of HPLC Agilent Technologies.
FPLC methods such as affinity of protein A/G for IgG on Akta explorer systems.
Preparing chromatography beds, mainly affinity chromatography.
Peptide synthesis based on Fmoc strategy.
Developing analytical methods for study protein-protein, peptide-protein interactions.
Knowledge of GMP and GLP systems.
Participation in qualification of systems – qualification performance: IQ, OQ, PQ according to GMP system.
Managment and participation in qualification of analytical methods according to GMP, GLP system.
Participation in creating CTD documentation.
Contract negotiation with suppliers of raw materials, equipment and systems.
Work on the sterile production of monoclonal antibodies in a pilot industrial scale in the technology of disposable systems and in the GMP system.
Knowledge of chromatographic systems and chromatographic media, packaging and qualification of chromatographic columns.

Analytical methods for water quality.